Preclinical investigations in postoperative pain: where are we now at ANS Biotech?
From theory….
Postoperative pain (POP) is defined as a transient but extremely strong activation of the nociceptive network. Mechanical hyperalgesia is observed by applying force directly on the wounds of patients after various surgical procedures such as inguinal herniorrhaphy, open cholecystectomy and abdominal hysterectomy.
At a preclinical stage, the gold standard for POP is the Brennan model (incisional pain). Nociceptive behaviors can be measured after incisional procedure, using notably the electronic Von Frey test.
The withdrawal threshold induced by the application of a progressive pressure is markedly decreased after the plantar incision and induced mechanical allodynia lasts for several days.
…to practice!
Historically, ANS Biotech offers the incisional pain model in rats.
For several reasons related to your pain program, you:
– can be restricted to the use of mice (target specificity)
– can face with low available amount of compound
– could need to access to transgenic strains
To support your needs, upon our tremendous experience in rats, we have developed the incisional pain model in mice.
In the standard experimental conditions, twenty four hours after surgery, the skin incision produces a mechanical allodynia which can be quantified using the electronic Von Frey test. In this test, an increasing pressure is applied onto either injured and control hindpaws and the paw withdrawal threshold is measured.
Assessment of the mechanical allodynia induced in the postoperative pain model in mice
The incisional pain model allows the assessment of the mechanisms of increased mechanical sensitivity following a surgical incision and is widely used for revealing the potency of new analgesics in this field.
To know more about ANS Biotech capabilities, do not hesitate to contact us!